Open AccessAutomated high-throughput light-sheet fluorescence microscopy of larval zebrafish
Author(s) -
Savannah L. Logan,
Christopher Dudley,
Ryan Baker,
Michael J. Taormina,
Edouard A. Hay,
Raghuveer Parthasarathy
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0198705
Subject(s) - light sheet fluorescence microscopy , microscopy , fluorescence microscope , throughput , fluorescence lifetime imaging microscopy , fluorescence , zebrafish , microscope , optics , biomedical engineering , materials science , biophysics , biology , computer science , physics , medicine , telecommunications , biochemistry , gene , wireless
Light sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation. Here, we describe an automated high-throughput light sheet fluorescence microscope in which specimens are positioned by and imaged within a fluidic system integrated with the sheet excitation and detection optics. We demonstrate the ability of the instrument to rapidly examine live specimens with minimal manual intervention by imaging fluorescent neutrophils over a nearly 0.3 mm 3 volume in dozens of larval zebrafish. In addition to revealing considerable inter-individual variability in neutrophil number, known previously from labor-intensive methods, three-dimensional imaging allows assessment of the correlation between the bulk measure of total cellular fluorescence and the spatially resolved measure of actual neutrophil number per animal. We suggest that our simple experimental design should considerably expand the scope and impact of light sheet imaging in the life sciences.