Open AccessCirculating proteomic patterns in AF related left atrial remodeling indicate involvement of coagulation and complement cascade
Author(s) -
Jelena Kornej,
Petra Büttner,
Elke Hammer,
Beatrice Engelmann,
Borislav Dinov,
Philipp Sommer,
Daniela Husser,
Gerhard Hindricks,
Uwe Völker,
Andreas Bollmann
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0198461
Subject(s) - atrial fibrillation , medicine , proteomics , complement system , proteome , cardiology , inflammation , catheter ablation , coagulation cascade , thrombin , bioinformatics , platelet , immune system , immunology , biology , biochemistry , gene
Background Left atrial (LA) electro-anatomical remodeling and diameter increase in atrial fibrillation (AF) indicates disease progression and is associated with poor therapeutic success. Furthermore, AF leads to a hypercoagulable state, which in turn promotes the development of a substrate for AF and disease progression in the experimental setting. The aim of this study was to identify pathways associated with LA remodeling in AF patients using untargeted proteomics approach. Methods Peripheral blood samples of 48 patients (62±10 years, 63% males, 59% persistent AF) undergoing AF catheter ablation were collected before ablation. 23 patients with left atrial low voltage areas (LVA), defined as <0.5 mV, and 25 patients without LVA were matched for age, gender and CHA 2 DS 2 -VASc score. Untargeted proteome analysis was performed using LC-ESI-Tandem mass spectrometry in a label free intensity based workflow. Significantly different abundant proteins were identified and used for pathway analysis and protein-protein interaction analysis. Results Analysis covered 280 non-redundant circulating plasma proteins. The presence of LVA correlated with 30 differentially abundant proteins of coagulation and complement cascade (q<0.05). Conclusions This pilot proteomic study identified plasma protein candidates associated with electro-anatomical remodeling in AF and pointed towards an imbalance in coagulation and complement pathway, tissue remodeling and inflammation.