Open AccessTrunk surface agarwood-inducing technique with Rigidoporus vinctus: An efficient novel method for agarwood production
Author(s) -
Xuyu Chen,
Yangyang Liu,
Yun Yang,
Jian Feng,
Peiwei Liu,
Chun Sui,
Jianhe Wei
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0198111
Subject(s) - agarwood , agar , biology , botany , horticulture , food science , traditional medicine , medicine , bacteria , alternative medicine , pathology , genetics
Only when Aquilaria spp. or Gyrinops spp. trees are wounded, due to insect attack, or microbial invasion, agarwood can be successfully induced. In the present study, a fungus which can induce agarwood formation efficiently was isolated and a suitable method for its application to induce agarwood formation was developed. Rigidoporus vinctus was isolated from the inner layers from infectious A . sinensis trees. When the fermentation liquid of fungi inoculated back to A . sinensis tree, agarwood was found to be induced. In addition, a novel method called trunk surface agarwood-inducing technique (Agar-Sit) was developed to produce agarwood with R . vinctus . The alcohol soluble extract content of the agarwood, up to 38.9%, far higher than the requirement (10%) in Chinese Pharmacopoeia and the six characteristic compounds of agarwood used as Chinese Medicinal Materials were all detected. Their relative percentages of the sesquiterpenes in the essential oil were 22.76%. This is the first report of the Agar-Sit and also the application of R . vinctus in agarwood induction. According to the results, when the combination of Agar-Sit and R . vinctus is used agarwood can be induced with high yield and good quality.