Open AccessDetermination of red blood cell deformability using centrifugal force in a three-dimensional-printed mini-disk (3D-PMD)
Author(s) -
Hyunjung Lim,
Seung Min Back,
Jeonghun Nam,
Hyuk Choi
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0197619
Subject(s) - fluidics , biomedical engineering , centrifugal force , microfluidics , materials science , rotation (mathematics) , 3d printing , red blood cell , spinning , composite material , rotational speed , mechanical engineering , nanotechnology , computer science , chemistry , electrical engineering , medicine , engineering , biochemistry , artificial intelligence
Measuring red blood cell (RBC) deformability has become important for clinical disease diagnostics. Various methods for measuring RBC deformability have been developed; however, they require costly and large instruments, long measuring time, and skilled personnel. In this study, we present a three-dimensional-printed mini-disk (3D-PMD) for measuring RBC deformability to overcome the previous limitations. For a miniaturized and low-cost setup, the 3D-PMD was fabricated by a 3D printing technique, which had not yet been used for fabricating a lab-on-a-compact disk (LOCD). Using a 3D printing technique, a multi-layered fluidic channel on the mini CD could be fabricated easily. During rotation by a spinning motor, the difference of the length of compressed RBCs in the fluidic channel was measured and analysed as compressibility indices (CIs) of normal and glutaraldehyde-treated hardened RBCs. The rotation speed and time were decided as 3000 rpm and 30 min, respectively, at which the difference of CI values between normal and hardened RBCs was largest (CI normal -CI hardened = 0.195).