Open AccessAFLP-based genetic diversity of wild orchardgrass germplasm collections from Central Asia and Western China, and the relation to environmental factors
Author(s) -
Chenglin Zhang,
Ming Sun,
Xinquan Zhang,
Shiyong Chen,
Gang Nie,
Yan Peng,
Linkai Huang,
Xiao Ma
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0195273
Subject(s) - genetic diversity , germplasm , biology , gene flow , genetic structure , dactylis glomerata , diversity index , ecology , upgma , genetic variation , plateau (mathematics) , botany , population , species richness , genetics , demography , sociology , mathematical analysis , poaceae , mathematics , gene
Dactylis glomerata L. (orchardgrass) is an important perennial forage species in temperate areas of the world. It is usually used for silage, grazing and hay because of its high nutritional value and reproducibility. Central Asia, Xinjiang and Tibetan Plateau in China possess various special micro-environments that harbor many valuable resources, while different degrees of degradation of the grassland ecosystem occurred due to climatic changing and human activities. Investigating the genetic diversity of wild D . glomerat could provide basis for collection, protection, and utilization of some excellent germplasm resources. Totally 210 individuals from 14 populations—five from Xinjiang, two from Kangding (Tibetan Plateau), and seven from Central Asia were identified using AFLP technology. The average values of Nei’s genetic diversity ( H j ) and Shannon information index ( H o ) were 0.383 and 0.394 respectively. UPGMA tree, STRUCTURE analysis and principal coordinate analysis (PCoA) showed populations from same region clustered together. AMOVA revealed 35.10% of the genetic differentiation ( F st ) occurred among populations. Gene flow ( N m ) was limited among all populations. Genetic diversity of D . glomerata was high but limited under isolation-by-distance pattern, resulting in high genetic differentiation and low gene flow among populations. Adjacent regions also exhibited similar results because of the barriers of high mountains. The environmental factors, such as precipitation, elevation, latitude and longitude also had some impacts on genetic diversity and structure pattern of populations.