Open AccessVCAM-1 expression is upregulated by CD34+/CD133+-stem cells derived from septic patients
Author(s) -
Christian Patry,
Christoph Remmé,
Christian Betzen,
Burkhard Tönshoff,
Benito A. Yard,
Grietje Beck,
Neysan Rafat
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0195064
Subject(s) - cd34 , stem cell , homing (biology) , progenitor cell , e selectin , cell adhesion molecule , immunology , cancer research , biology , medicine , cell adhesion , microbiology and biotechnology , cell , biochemistry , ecology
CD34 + /CD133 + - cells are a bone marrow derived stem cell population, which presumably contain vascular progenitor cells and are associated with improved vascular repair. In this study, we investigated whether the adhesion molecules ICAM-1 (intercellular adhesion molecule-1), VCAM-1 (vascular adhesion molecule-1), E-selectin und L-selectin, which are involved in homing of vascular stem cells, are upregulated by CD34 + /CD133 + -stem cells from septic patients and would be associated with improved clinical outcome. Peripheral blood mononuclear cells from intensive care unit (ICU) patients with (n = 30) and without sepsis (n = 10), and healthy volunteers (n = 15) were isolated using Ficoll density gradient centrifugation. The expression of VCAM-1, ICAM-1, E-selectin and L-selectin was detected on CD34 + /CD133 + -stem cells by flow cytometry. The severity of disease was assessed by the Simplified Acute Physiology Score (SAPS) II. Serum concentrations of vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-2 were determined by Enzyme-linked immunosorbent assay . The expression of VCAM-1, ICAM-1, E-selectin and L-selectin by CD34 + /CD133 + -stem cells was significantly upregulated in septic patients, and correlated with sepsis severity. Furthermore, high expression of VCAM-1 by CD34 + /CD133 + -stem cells revealed a positive association with mortalitiy (p<0.05). Furthermore, significantly higher serum concentrations of VEGF and Ang-2 were found in septic patients, however none showed a strong association with survival. Our data suggest, that VCAM-1 upregulation on CD34 + /CD133 + -stem cells could play a crucial role in their homing in the course of sepsis. An increase in sepsis severity resulted in both and increase in CD34 + /CD133 + -stem cells and VCAM-1-expression by those cells, which might reflect an increase in need for vascular repair.