Open AccessRice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter
Author(s) -
Won Tae Yang,
Dongwon Baek,
DaeJin Yun,
Kwang-Sik Lee,
So Yeon Hong,
Ki Deuk Bae,
Young Soo Chung,
Yong Sham Kwon,
Du Hyun Kim,
KiHong Jung,
Doh Hoon Kim
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0194628
Subject(s) - arabidopsis , myb , biology , genetically modified rice , oryza sativa , transcription factor , arabidopsis thaliana , microbiology and biotechnology , shoot , transcription (linguistics) , meristem , transgene , gene , genetically modified crops , botany , biochemistry , mutant , linguistics , philosophy
Myeloblastosis (MYB) transcription factors play central roles in plant developmental processes and in responses to nutrient deficiency. In this study, OsMYB5P, an R2R3-MYB transcription factor, was isolated and identified from rice ( Oryza sativa L. ‘Dongjin’) under inorganic phosphate (Pi)-deficient conditions. OsMYB5P protein is localized to the nucleus and functions as a transcription activator in plant development. Overexpression of OsMYB5P in rice and Arabidopsis ( Arabidopsis thaliana Col-0) increases tolerance to phosphate starvation, whereas OsMYB5P knock-out through RNA interference increases sensitivity to Pi depletion in rice. Furthermore, shoots and roots of transgenic rice plants overexpressing OsMYB5P were longer than those of wild plants under both normal and Pi-deficient conditions. These results indicate that OsMYB5P is associated with the regulation of shoot development and root- system architecture. Overexpression of OsMYB5P led to increased Pi accumulation in shoots and roots. Interestingly, OsMYB5P directly bound to MBS (MYB binding site) motifs on the OsPT5 promoter and induced transcription of OsPT5 in rice. In addition, overexpression of OsMYB5P in Arabidopsis triggered increased expression of AtPht1;3 , an Arabidopsis Pi transporter, in shoots and roots under normal and Pi-deficient conditions. Together, these results demonstrate that overexpression of OsMYB5P increases tolerance to Pi deficiency in plants by modulating Pi transporters at the transcriptional level in monocots and dicots.