Open AccessBeware the intruder: Real time observation of infiltrated neutrophils and neutrophil—Microglia interaction during stroke in vivo
Author(s) -
Jens Neumann,
Sophie Henneberg,
Susanne von Kenne,
N. S. Nolte,
Andreas J. Müller,
Burkhart Schraven,
Michael Görtler,
Klaus G. Reymann,
Matthias Gunzer,
Monika RiekBurchardt
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0193970
Subject(s) - microglia , parenchyma , inflammation , infiltration (hvac) , immune system , intravital microscopy , in vivo , bone marrow , pathology , immunology , medicine , innate immune system , granulocyte , stroke (engine) , biology , physics , microbiology and biotechnology , thermodynamics , mechanical engineering , engineering
Inflammation plays an important role in the pathogenesis of ischemic stroke including an acute and prolonged inflammatory process. The role of neutrophil granulocytes as first driver of the immune reaction from the blood site is under debate due to controversial findings. In bone marrow chimeric mice we were able to study the dynamics of tdTomato-expressing neutrophils and GFP-expressing microglia after photothrombosis using intravital two-photon microscopy. We demonstrate the infiltration of neutrophils into the brain parenchyma and confirm a long-lasting contact between neutrophils and microglia as well as an uptake of neutrophils by microglia clearing the brain from peripheral immune cells.