Open AccessInhibitor of apoptosis proteins, NAIP, cIAP1 and cIAP2 expression during macrophage differentiation and M1/M2 polarization
Author(s) -
Virginia Morón-Calvente,
Salvador Romero-Pinedo,
Sofía Toribio-Castelló,
Julio PlazaDíaz,
Ana Clara AbadíaMolina,
Domingo I. Rojas-Barros,
Shawn T. Beug,
Eric C. LaCasse,
Alex MacKenzie,
Robert G. Korneluk,
Francisco Abadía-Molina
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0193643
Subject(s) - biology , inhibitor of apoptosis , microbiology and biotechnology , phenotype , downregulation and upregulation , innate immune system , macrophage , macrophage polarization , acquired immune system , immune system , apoptosis , immunology , genetics , gene , programmed cell death , in vitro
Monocytes and macrophages constitute the first line of defense of the immune system against external pathogens. Macrophages have a highly plastic phenotype depending on environmental conditions; the extremes of this phenotypic spectrum are a pro-inflammatory defensive role (M1 phenotype) and an anti-inflammatory tissue-repair one (M2 phenotype). The Inhibitor of Apoptosis (IAP) proteins have important roles in the regulation of several cellular processes, including innate and adaptive immunity. In this study we have analyzed the differential expression of the IAPs, NAIP, cIAP1 and cIAP2, during macrophage differentiation and polarization into M1 or M2. In polarized THP-1 cells and primary human macrophages, NAIP is abundantly expressed in M2 macrophages, while cIAP1 and cIAP2 show an inverse pattern of expression in polarized macrophages, with elevated expression levels of cIAP1 in M2 and cIAP2 preferentially expressed in M1. Interestingly, treatment with the IAP antagonist SMC-LCL161, induced the upregulation of NAIP in M2, the downregulation of cIAP1 in M1 and M2 and an induction of cIAP2 in M1 macrophages.