Open AccessDevelopment of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed
Author(s) -
Mi Jin Lee,
Ho Jin Kim
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0193584
Subject(s) - chromatography , formic acid , chemistry , mass spectrometry , detection limit , liquid chromatography–mass spectrometry , analytical chemistry (journal)
A novel and simple method for detecting 6 zearalenones in animal feed using liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) and immunoaffinity columns (IAC) was developed. The chromatographic peaks of the 6 zearalenones were successfully identified by comparing their retention times and mass spectrum with reference standards. The mobile phase was composed of mobile phase A (water) and B (0.5% formic acid in ACN). Method validation was performed with linearity, sensitivity, selectivity, accuracy and precision. The limits of detection (LODs) for the instrument used to study zearalenones ranged from 0.3 to 1.1 μg/kg, and the limits of quantification (LOQs) ranged from 1.0 to 2.2 μg/kg. Average recoveries of the 6 zearalenones ranged from 82.5% to 106.4%. Method replication resulted in intra-day and inter-day peak area variation of <3.8%. The developed method was specific and reliable and is suited for the routine analysis of zearalenones in animal feed.