Open AccessCytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight
Author(s) -
Irina V. Ogneva,
Sergey S. Loktev,
Vladimir Sychev
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0192643
Subject(s) - demethylase , dna methylation , biology , messenger rna , epigenetics , histone , cytoskeleton , histone deacetylase , microbiology and biotechnology , gene expression , methylation , epigenomics , dna , gene , genetics , cell
The purpose of this work was to evaluate the protein and mRNA expression levels of multiple cytoskeletal proteins in the cardiac and lung tissue of mice that were euthanized onboard the United States Orbital Segment of the International Space Station 37 days after the start of the SpaceX-4 mission (September 2014, USA). The results showed no changes in the cytoskeletal protein content in the cardiac and lung tissue of the mice, but there were significant changes in the mRNA expression levels of the associated genes, which may be due to an increase in total genome methylation. The mRNA expression levels of DNA methylases, the cytosine demethylases Tet1 and Tet3, histone acetylase and histone deacetylase did not change, and the mRNA expression level of cytosine demethylase Tet2 was significantly decreased.