Open AccessThe effect of S100A6 on nuclear translocation of CacyBP/SIP in colon cancer cells
Author(s) -
Shanshan Feng,
Qiaozhi Zhou,
Bo Yang,
Qianqian Li,
Aiqin Liu,
Yingying Zhao,
Qiu Chang-qing,
Jun Ge,
Huihong Zhai
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0192208
Subject(s) - ionomycin , chromosomal translocation , immunoprecipitation , gene knockdown , microbiology and biotechnology , biology , cytosol , nuclear localization sequence , transfection , chemistry , intracellular , cancer research , cell culture , nucleus , antibody , biochemistry , immunology , genetics , gene , enzyme
Background Calcyclin Binding Protein/(Siah-1 interacting protein) (CacyBP/SIP) acts as an oncogene in colorectal cancer. The nuclear accumulation of CacyBP/SIP has been linked to the proliferation of cancer cells. It has been reported that intracellular Ca 2+ induces the nuclear translocation of CacyBP/SIP. However, the molecular mechanism of CacyBP/SIP nuclear translocation has yet to be elucidated. The purpose of this study was to test whether the Ca 2+ -dependent binding partner S100 protein is involved in CacyBP/SIP nuclear translocation in colon cancer SW480 cells. Methods The subcellular localization of endogenous CacyBP/SIP was observed following the stimulation of ionomycin or BAPTA/AM by immunofluorescence staining in SW480 cells. S100A6 small interfering RNAs (siRNA) were transfected into SW480 cells. Immunoprecipitation assays detected whether S100 protein is relevant to the nuclear translocation of CacyBP/SIP in response to changes in [Ca 2+ ] i . Results We observed that endogenous CacyBP/SIP is translocated from the cytosol to the nucleus following the elevation of [Ca 2+ ] i by ionomycin in SW480 cells. Co-immunoprecipitation experiments showed that the interaction between S100A6 and CacyBP/SIP was increased simultaneously with elevated Ca 2+ . Knockdown of S100A6 abolished the Ca 2+ effect on the subcellular translocation of CacyBP/SIP. Conclusion Thus, we demonstrated that S100A6 is required for the Ca 2+ -dependent nuclear translocation of CacyBP/SIP in colon cancer SW480 cells.