Open AccessThe effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis
Author(s) -
Maïté Van Cauter,
Olivier Cornu,
JeanCyr Yombi,
Hector Rodríguez-Villalobos,
Ludovic Kaminski
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0192048
Subject(s) - staphylococcus epidermidis , contamination , staphylococcus aureus , microbiology and biotechnology , microorganism , medicine , staphylococcus , bacteria , food science , biology , ecology , genetics
Background Prosthetic Joint Infection (PJI) is a rare but devastating complications with high morbitity and mortality. The identification of the causal microorganism remains crucial and determines therapeutic strategies and success. Microbiology cultures remain the common method to diagnose PJI. Unfortunately, 14% of intra-articular punctures remain negative after culture. The microorganisms are best detected by inoculation of microbiology samples in blood culture bottles (Bactec), or after sonication of the implant and polymerase chain reaction (PCR). The identification of the causal microorganism remains crucial and determines therapeutic success. Objectives This study was conducted to assess the effect of culture lead time and sample storage temperature on the detection of the pathogen. Methods We obtained bone fragments from femoral heads during primary arthroplasty. Bone fragments were contaminated with a strain of Staphylococcus epidermidis . Four set-ups with different combinations of storage delay and storage temperature were tested. Results Our study shows the need to cultivate as soon as possible and optimally within 2h after the completion of sampling. Temporary storage in a refrigerator at 4°C also appears to have a positive influence on bacterial viability. At present, these conclusions concern only the Staphylococcus Epidermidis . Others studies are requested to generalize this conclusion to other bacteria.