Open AccessSurface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores
Author(s) -
Xixi Dai,
Minggang Liu,
Kangcheng Pan,
Jing Yang
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0191627
Subject(s) - bacillus subtilis , microbiology and biotechnology , salmonella , spore , recombinant dna , serotype , biology , antigen , immunofluorescence , immunization , antibody , bacteria , immunology , gene , biochemistry , genetics
Salmonellosis is a major public health problem throughout the world. Thus, there is a huge need for diversified control strategies for Salmonella infections. In this work, we have assessed the potential use of Bacillus subtilis ( B . subtilis ) spores for the expression of a major protective antigen of Salmonella serovar Pullorum, OmpC. The expression of OmpC on the surface of spores was determined by immunofluorescence microscopy. Mice immunized with recombinant spores expressing the OmpC antigen presented significant levels of OmpC-specific serum IgG and mucosal SIgA antibodies than in mice immunized with non-recombinant spores ( p <0.01). In addition, oral immunization with recombinant spores was able to induce a significant level of protection in mice against lethal challenge with Salmonella serovar Typhimurium. These results suggest that B . subtilis spores have promising potential in the development of mucosal vaccines against Salmonella infections.