Intranasal vaccination with M2e5x virus-like particles induces humoral and cellular immune responses conferring cross-protection against heterosubtypic influenza viruses | Zendy

YoungTae Lee | Zendy; EunJu Ko | Zendy; Youri Lee | Zendy; KiHye Kim | Zendy; Minchul Kim | Zendy; Yuna Lee | Zendy; SangMoo Kang | Zendy

Open Access

Intranasal vaccination with M2e5x virus-like particles induces humoral and cellular immune responses conferring cross-protection against heterosubtypic influenza viruses

Author(s) -

YoungTae Lee,

EunJu Ko,

Youri Lee,

KiHye Kim,

Minchul Kim,

Yuna Lee,

SangMoo Kang

Publication year - 2018

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0190868

Subject(s) - vaccination , immune system , nasal administration , virology , influenza a virus , virus , immunology , germinal center , biology , viral matrix protein , cd8 , antibody , b cell

Current influenza vaccines do not provide broad cross-protection. Here, we report that intranasal vaccination with virus-like particles containing the highly conserved multiple ectodomains of matrix protein 2 (M2e5x VLP) of influenza virus induces broad cross-protection by M2-specific humoral and cellular immune responses. M2e5x VLP intranasal vaccination prevented severe weight loss, attenuated inflammatory cytokines and cellular infiltrates, and lowered viral loads, and induced germinal center phenotypic B and plasma cells. In addition, depletion studies demonstrate the protective roles of CD4 and CD8 T cells induced by M2e5x VLP intranasal vaccination. Thus, this study provides evidence that mucosal delivery of M2e5x VLP vaccine provides cross-protection by inducing humoral and cellular immune responses.

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