Open AccessMicroRNAs miR-19, miR-340, miR-374 and miR-542 regulate MID1 protein expression
Author(s) -
Kristoffer Unterbruner,
Frank Matthes,
Judith Schilling,
Rohit Nalavade,
Stephanie Weber,
Jennifer Winter,
Sybille Krauß
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0190437
Subject(s) - microrna , biology , translation (biology) , untranslated region , ubiquitin ligase , three prime untranslated region , downregulation and upregulation , messenger rna , pi3k/akt/mtor pathway , regulation of gene expression , microbiology and biotechnology , signal transduction , bioinformatics , ubiquitin , genetics , gene
The MID1 ubiquitin ligase activates mTOR signaling and regulates mRNA translation. Misregulation of MID1 expression is associated with various diseases including midline malformation syndromes, cancer and neurodegenerative diseases. While this indicates that MID1 expression must be tightly regulated to prevent disease states specific mechanisms involved have not been identified. We examined miRNAs to determine mechanisms that regulate MID1 expression. MicroRNAs (miRNA) are small non-coding RNAs that recognize specific sequences in their target mRNAs. Upon binding, miRNAs typically downregulate expression of these targets. Here, we identified four miRNAs, miR-19, miR-340, miR-374 and miR-542 that bind to the 3’-UTR of the MID1 mRNA. These miRNAs not only regulate MID1 expression but also mTOR signaling and translation of disease associated mRNAs and could therefore serve as potential drugs for future therapy development.