Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle

Listeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L . monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catfish fillet attachment was investigated. To achieve this, boron-based small molecules designed to interfere with the active site of the L . monocytogenes LapB protein were developed, and their ability to prevent L . monocytogenes attachment to fish fillet was tested. Results indicated that seven out of nine different small molecules were effective in reducing the Listeria attachment to catfish fillets. Of these, three small molecules (SM3, SM5, and SM7) were highly effective in blocking Listeria attachment to catfish fillets. This study suggests an alternative strategy for reduction of L . monocytogenes contamination in fresh and frozen fish products.