Open AccessFirst insights into a type II toxin-antitoxin system from the clinical isolate Mycobacterium sp. MHSD3, similar to epsilon/zeta systems
Author(s) -
Daniel JaénLuchoro,
Francisco Aliaga-Lozano,
Rosa M. Gomila,
Margarita Gomila,
Francisco SalvàSerra,
Jorge Lalucat,
Antonio Bennasar
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0189459
Subject(s) - antitoxin , escherichia coli , microbiology and biotechnology , mycobacterium chelonae , biology , toxin , genome , gene , mycobacterium , genetics , bacteria
A putative type II toxin-antitoxin (TA) system was found in the clinical isolate Mycobacterium sp. MHSD3, a strain closely related to Mycobacterium chelonae . Further analyses of the protein sequences of the two genes revealed the presence of domains related to a TA system. BLAST analyses indicated the presence of closely related proteins in the genomes of other recently published M . chelonae strains. The functionality of both elements of the TA system was demonstrated when expressed in Escherichia coli cells, and the predicted structure of the toxin is very similar to those of well-known zeta-toxins, leading to the definition of a type II TA system similar to epsilon/zeta TA systems in strains that are closely related to M . chelonae .