Open AccessFlow cytometric discrimination of seven lineage markers by using two fluorochromes
Author(s) -
Francesco Boin,
Maria Letizia Giardino Torchia,
Ivan Borrello,
Kimberly Noonan,
Matthew S. Neil,
Mark J. Soloski,
Raffaello Cimbro
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0188916
Subject(s) - lineage (genetic) , biology , flow cytometry , genetics , gene
Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4 + and CD8 + T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.