Flow cytometric discrimination of seven lineage markers by using two fluorochromes | Zendy

Francesco Boin | Zendy; Maria Letizia Giardino Torchia | Zendy; Ivan Borrello | Zendy; Kimberly Noonan | Zendy; Matthew S Neil | Zendy; Mark J. Soloski | Zendy; Raffaello Cimbro | Zendy

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Flow cytometric discrimination of seven lineage markers by using two fluorochromes

Author(s) -

Francesco Boin,

Maria Letizia Giardino Torchia,

Ivan Borrello,

Kimberly Noonan,

Matthew S Neil,

Mark J. Soloski,

Raffaello Cimbro

Publication year - 2017

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0188916

Subject(s) - lineage (genetic) , biology , flow cytometry , genetics , gene

Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4 + and CD8 + T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.

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