Open AccessChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction
Author(s) -
Hiba Baaziz,
Cyril Gambari,
Anne Boyeldieu,
Amine Ali Chaouche,
Radia Alatou,
Vincent Méjean,
Cécile Jourlin-Castelli,
Michel Fons
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0188516
Subject(s) - shewanella oneidensis , chromate conversion coating , efflux , potassium chromate , chemistry , mutant , microbiology and biotechnology , biology , bacteria , gene , biochemistry , genetics , chromium , organic chemistry
The chromate efflux pump encoding gene chrA SO was identified on the chromosome of Shewanella oneidensis MR1. Although chrA SO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant Δ chrA SO exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrA SO in E . coli confers resistance to high chromate concentration. Moreover, expression of chrA SO in S . oneidensis and E . coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction.