T cell recognition of Mycobacterium tuberculosis peptides presented by HLA-E derived from infected human cells | Zendy

Curtis McMurtrey | Zendy; Melanie J. Harriff | Zendy; Gwendolyn Swarbrick | Zendy; Amanda Duncan | Zendy; Meghan Cansler | Zendy; Megan | Zendy; Wilfried Bardet | Zendy; Kenneth W. Jackson | Zendy; Deborah A. Lewinsohn | Zendy; William H. Hildebrand | Zendy; David Lewinsohn | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

T cell recognition of Mycobacterium tuberculosis peptides presented by HLA-E derived from infected human cells

Author(s) -

Curtis McMurtrey,

Melanie J. Harriff,

Gwendolyn Swarbrick,

Amanda Duncan,

Meghan Cansler,

Megan,

Wilfried Bardet,

Kenneth W. Jackson,

Deborah A. Lewinsohn,

William H. Hildebrand,

David Lewinsohn

Publication year - 2017

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0188288

Subject(s) - mycobacterium tuberculosis , human leukocyte antigen , cd8 , biology , major histocompatibility complex , tuberculosis , t cell , t cell receptor , peptide , antigen , cytotoxic t cell , epitope , immunology , immune system , virology , microbiology and biotechnology , medicine , biochemistry , in vitro , pathology

HLA-E is a non-conventional MHC Class I molecule that has been recently demonstrated to present pathogen-derived ligands, resulting in the TCR-dependent activation of αβ CD8 + T cells. The goal of this study was to characterize the ligandome displayed by HLA-E following infection with Mycobacterium tuberculosis (Mtb) using an in-depth mass spectrometry approach. Here we identified 28 Mtb ligands derived from 13 different source proteins, including the Esx family of proteins. When tested for activity with CD8 + T cells isolated from sixteen donors, nine of the ligands elicited an IFN-γ response from at least one donor, with fourteen of 16 donors responding to the Rv0634A 19-29 peptide. Further evaluation of this immunodominant peptide response confirmed HLA-E restriction and the presence of Rv0634A 19-29 -reactive CD8 + T cells in the peripheral blood of human donors. The identification of an Mtb HLA-E ligand that is commonly recognized may provide a target for a non-traditional vaccine strategy.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research