Open AccessA new approach by optical coherence tomography for elucidating biofilm formation by emergent Candida species
Author(s) -
Melyna Chaves Leite de Andrade,
Marcos A. Soares de Oliveira,
Franz de Assis Graciano dos Santos,
Pamella de Brito Ximenes Vilela,
Michellangelo Nunes da Silva,
Danielle Patrícia Cerqueira Macêdo,
Reginaldo Gonçalves de Lima-Neto,
Henrique Jonh Pereira Neves,
Ildnay de Souza Lima Brandão,
Guilherme Maranhão Chaves,
Renato E. de Araujo,
Rejane Pereira Neves
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0188020
Subject(s) - biofilm , context (archaeology) , optical coherence tomography , microbiology and biotechnology , biology , metabolic activity , bacteria , chemistry , biophysics , biological system , optics , genetics , physics , paleontology
The majority of microorganisms present a community lifestyle, establishing biofilm ecosystems. However, little is known about its formation in emergent Candida species involved in catheter-related infections. Thus, various techniques may be used in the biofilm detection to elucidate structure and clinical impact. In this context, we report the ability of emergent Candida species ( Candida haemulonii , C . lusitaniae , C . pelliculosa , C . guilliermondii , C . famata and C . ciferrii ) on developing well structured biofilms with cell viability and architecture, using optical coherence tomography (OCT). This new approach was compared with XTT analyses and Scanning Electron Microscopy (SEM). A positive correlation between oxidative activity (XTT) and OCT results (r = 0.8752, p < 0.0001) was observed. SEM images demonstrated cells attachment, multilayer and morphologic characteristics of the biofilm structure. C . lusitaniae was the emergent species which revealed the highest scattering extension length and oxidative metabolism when evaluated by OCT and XTT methods, respectively. Herein, information on C . ciferri biofilm structure were presented for the first time. The OCT results are independently among Candida strains and no species-specific pattern was observed. Our findings strongly contribute for clinical management based on the knowledge of pathogenicity mechanisms involving emergent yeasts.