Open AccessProteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection
Author(s) -
Mei-Guey Lei,
Ravi Gupta,
Chia Y. Lee
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0187981
Subject(s) - biofilm , staphylococcus aureus , microbiology and biotechnology , extracellular matrix , immune system , proteomics , matrix (chemical analysis) , biology , staphylococcal infections , viral matrix protein , chemistry , immunology , bacteria , biochemistry , gene , genetics , chromatography , virus
The matrix proteins of Staphylococcus aureus biofilm have not been well defined. Previous efforts to identify these proteins were performed using in vitro systems. Here we use a proteomic approach to identify biofilm matrix proteins directly from infected bone implants using a rat model of orthopedic implant-associated S . aureus infection. Despite heavy presence of host proteins, a total of 28 and 105 S . aureus proteins were identified during acute infection and chronic infection, respectively. Our results show that biofilm matrix contains mostly intracellular cytoplasmic proteins and, to a much less extent, extracellular and cell surface-associated proteins. Significantly, leukocidins were identified in the biofilm matrix during chronic infection, suggesting S . aureus is actively attacking the host immune system even though they are protected within the biofilm. The presence of two surface-associated proteins, Ebh and SasF, in the infected bone tissue during acute infection was confirmed by immunohistochemistry. In addition, a large number of host proteins were found differentially expressed in response to S . aureus biofilm formed on bone implants.