Open AccessThe infection and impact of Azorhizobium caulinodans ORS571 on wheat (Triticum aestivum L.)
Author(s) -
Huawei Liu,
Xiaojing Wang,
Qi HuaiTing,
Qian Wang,
Yongchao Chen,
Qiang Li,
Yuying Zhang,
Li Qiu,
Julia Elise Fontana,
Baohong Zhang,
Weiling Wang,
Yibing Xie
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0187947
Subject(s) - rhizobia , germination , biology , agronomy , rhizobium , crop , sowing , symbiosis , horticulture , inoculation , bacteria , genetics
Based on our previous study, cereal crop wheat ( Triticum aestivum L.) could be infected by rhizobia Azorhizobium caulinodans ORS571, and form para-nodules with the induction of 2.4-dichlorophenoxyacetic acid, a common plant growth regulator. To enhance this infection and the potential agricultural application, we compared six different infection methods (Direct seed dip; Seed germination dip; Pruned-root dip; Foliar spray; Circum-soil dip; Seed dip and circum-soil dip) for achieving the high efficient infection of A . caulinodans into wheat plants by employing a green fluorescent protein (gfp) -labeled Azorhizobium caulinodans strain ORS571. With proper methods, copious rhizobia could enter the interior and promote the growth of wheat to the hilt. Circum-soil dip was proved to be the most efficient method, seed germination dip and pruned-root dip is the last recommended to infect wheat, seed germination dip and seed dip and circum-soil dip showed better effects on plant growth, pruned-root dip did not show too much effect on plant growth. This study laid the foundation for understanding the interaction between rhizobia and cereal crops and the growth-promoting function of rhizobia.