Open AccessDesign and synthesis of a fluorescent probe based on naphthalene anhydride and its detection of copper ions
Author(s) -
Guangjie He,
Chenxi Liu,
Xiaobo Liu,
Qingzhi Wang,
Aiying Fan,
Songjun Wang,
Xiangyang Qian
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0186994
Subject(s) - fluorescence , copper , detection limit , chemistry , metal ions in aqueous solution , photochemistry , selectivity , ion , inorganic chemistry , organic chemistry , chromatography , catalysis , physics , quantum mechanics
Copper, as the third most abundant transition metal ions of human, plays an essential role in the redox reaction, signal transduction, hematopoiesis, and other physiological processes. Abnormal content of copper ions in the body will cause some diseases such as anemia, coronary heart disease, Menkes’ syndrome. In this article, a new fluorescence probe L for Cu 2+ was designed and synthetized by using 4-bromo-1,8 naphthalene anhydride and 2-thiophene formaldehyde as raw materials. Fluorescent probe L itself exhibited strong fluorescence, upon the addition of Cu 2+ ions, the fluorescence was quenched. The fluorescent detection limit for Cu 2+ ions was determined to be 1.8 μM based on a 3δ/S method. UV-vis absorption and fluorescence spectra indicated that probe L showed good selectivity and sensitivity for Cu 2+ , and this selectivity was not interfered by other metal ions and anions. Further cell fluorescence imaging experiments indicated that the probe L had potential to be used to examine copper ions in vivo.