Open AccessAged interleukin-10tm1Cgn chronically inflamed mice have substantially reduced fat mass, metabolic rate, and adipokines
Author(s) -
Reyhan Westbrook,
Huan Yang,
Jacqueline M. Langdon,
Cindy N. Roy,
Jin A. Kim,
Parichoy Pal Choudhury,
Xue Qin,
Andrea Di Francesco,
Rafa de Cabo,
Jeremy Walston
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0186811
Subject(s) - endocrinology , adipokine , medicine , adiponectin , lean body mass , respiratory quotient , leptin , inflammation , adipose tissue , obesity , biology , insulin resistance , body weight
Interleukin 10 tm1Cgn (IL 10 tm ) mice have been utilized as a model of chronic inflammation and declining health span because of their propensity to develop chronic activation in NFkB pathways, skeletal muscle and cardiac changes, and mitochondrial dysfunction. We hypothesized that older IL 10 tm frail mice would have alterations similar to frail, older humans in measured parameters of glucose metabolism, oxygen consumption (VO 2 ), respiratory quotient (RQ), spontaneous locomotor activity, body composition and plasma adipokine levels. To test this hypothesis, we investigated these metabolic parameters in cohorts of 3, 10, and 20 month old IL 10 tm female mice and age and gender matched C57Bl/6 mice. Insulin sensitivity, glucose homeostasis, locomotor activity and RQ were not significantly altered between the two strains of mice. Interestingly, old IL 10 tm mice had significantly decreased VO 2 when normalized by lean mass, but not when normalized by fat mass or the lean/fat mass ratio. NMR based body composition analysis and dissection weights show that fat mass is decreased with age in IL 10 tm mice compared to controls. Further, plasma adiponectin and leptin were also decreased in IL 10 tm .These findings suggest that frailty observed in this mouse model of chronic inflammation may in part be driven by alterations in fat mass, hormone secretion and energy metabolism.