Open AccessEvaluation of gastrointestinal bacterial population for the production of holocellulose enzymes for biomass deconstruction
Author(s) -
Dhaneshwaree Asem,
Vincent Vineeth Leo,
Ajit Kumar Passari,
Mary Vanlalhruaii Tonsing,
Jarina Joshi,
Sivakumar Uthandi,
Abeer Hashem,
Elsayed Fathi Abd_Allah,
Bhim Pratap Singh
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0186355
Subject(s) - biomass (ecology) , xylanase , food science , ruminant , cellulase , population , biology , fermentation , microorganism , lignocellulosic biomass , microbiology and biotechnology , bacteria , botany , agronomy , enzyme , biochemistry , genetics , demography , sociology , crop
The gastrointestinal (GI) habitat of ruminant and non-ruminant animals sustains a vast ensemble of microbes that are capable of utilizing lignocellulosic plant biomass. In this study, an indigenous swine (Zovawk) and a domesticated goat (Black Bengal) were investigated to isolate bacteria having plant biomass degrading enzymes. After screening and enzymatic quantification of eighty-one obtained bacterial isolates, Serratia rubidaea strain DBT4 and Aneurinibacillus aneurinilyticus strain DBT87 were revealed as the most potent strains, showing both cellulase and xylanase production. A biomass utilization study showed that submerged fermentation (SmF) of D2 (alkaline pretreated pulpy biomass) using strain DBT4 resulted in the most efficient biomass deconstruction with maximum xylanase (11.98 U/mL) and FPase (0.5 U/mL) activities (55°C, pH 8). The present study demonstrated that bacterial strains residing in the gastrointestinal region of non-ruminant swine are a promising source for lignocellulose degrading microorganisms that could be used for biomass conversion.