Open AccessEndosphere microbiome comparison between symptomatic and asymptomatic roots of Brassica napus infected with Plasmodiophora brassicae
Author(s) -
Ying Zhao,
Zhixiao Gao,
Binnian Tian,
Kai Bi,
Tao Chen,
Huiquan Liu,
Jiǎtāo Xiè,
Jiāsēn Chéng,
Yànpíng Fù,
Dàohóng Jiāng
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0185907
Subject(s) - clubroot , biology , brassica , microbiome , asymptomatic , phytoplasma , population , brassica rapa , botany , medicine , genetics , polymerase chain reaction , pathology , gene , restriction fragment length polymorphism , environmental health
Clubroot caused by Plasmodiophora brassicae , is a severe disease of cruciferous crops that causes large hypertrophic galls in the roots. The plant microbiome is important for growth promotion and disease suppression. In this study, using 16S rRNA and internal transcribed spacer (ITS) sequencing techniques, we compared the endosphere microbiome of symptomatic and asymptomatic B . napus roots infected with P . brassicae collected from the same natural clubroot field. The results showed that the microbial population and its relative abundance in the asymptomatic roots was far higher than that in the symptomatic roots, and that many microorganisms in asymptomatic roots have biological control and plant growth promotion functions that may be related to clubroot symptoms. These results suggest the importance of the endosphere microbiome in clubroot disease and provide potential bio-control resources for its prevention.