Open AccessDCs facilitate B cell responses against microbial DNA via DC-SIGN
Author(s) -
Joris K. Sprokholt,
Marieke H. Heineke,
Tanja M. Kaptein,
John L. van Hamme,
Teunis B. H. Geijtenbeek
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0185580
Subject(s) - dna , endosome , tlr9 , dc sign , microbiology and biotechnology , biology , immune system , receptor , extracellular , pattern recognition receptor , toll like receptor 9 , dendritic cell , innate immune system , gene , intracellular , biochemistry , immunology , gene expression , dna methylation
Microbial DNA is highly immunostimulatory and is sensed by endosomal pattern recognition receptors after release from internalized microbes. It is unclear how extracellular DNA released from dead microbes is delivered to endosomal PRRs to induce immune responses. Here we have investigated the ability of DCs to bind and internalize extracellular E . coli DNA as well as synthetic DNA. DCs internalized E . coli and synthetic DNA, which was dependent on the C-type lectin receptor DC-SIGN. Notably, endosomal uptake of DNA by DCs enhanced TLR9-dependent responses of B cells against DNA. Hence, we have identified DC-SIGN as a cell surface receptor for DNA that facilitates immune responses directed against DNA.