Open AccessA new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells
Author(s) -
Narcisa Martínez-Martínez,
Emma MartínezAlonso,
Mariona Tomàs,
Josef Neumüller,
Margit Pavelka,
José A. Martı́nez-Menárguez
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0185557
Subject(s) - golgi apparatus , endoplasmic reticulum , microbiology and biotechnology , organelle , secretory pathway , endocytic cycle , biology , cell type , ultrastructure , microtubule , cell , chemistry , anatomy , endocytosis , biochemistry
Principal epididymal cells have one of the largest and more developed Golgi complex of mammalian cells. In the present study, we have used this cell as model for the study of the three-dimensional architecture of the Golgi complex of highly secretory and endocytic cells. Electron tomography demonstrated the presence in this cell type of some unknown or very unusual Golgi structures such as branched cisternae, pocket-like cisternal invaginations or tubular connections. In addition, we have used this methodology and immunoelectron microscopy to analyze the close relationship between this organelle and both the endoplasmic reticulum and microtubules, and to describe in detail how these elements interact with compact and non-compact regions of the ribbon.