Open AccessSunflower oil supplementation affects the expression of miR-20a-5p and miR-142-5p in the lactating bovine mammary gland
Author(s) -
Lenha Mobuchon,
Sandrine Le Guillou,
Sylvain Marthey,
Johann Laubier,
Dénis Laloë,
Sébastien Bes,
Fabienne Le Provost,
Christine Leroux
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0185511
Subject(s) - mammary gland , microrna , biology , sunflower oil , lipid metabolism , gene expression , lactation , gene , sunflower , regulation of gene expression , endocrinology , medicine , microbiology and biotechnology , genetics , biochemistry , pregnancy , breast cancer , cancer , agronomy
Oil supplementation in dairy cattle diets is used to modulate milk fat composition, as well as the expression of mammary lipogenic genes, whose regulation remains unclear. MiRNAs are small non-coding RNA considered as crucial regulators of gene expression, offering clues to explain the mechanism underlying gene nutriregulation. The present study was designed to identify miRNAs whose expression in the cow mammary gland is modulated by sunflower oil supplementation. MiRNomes were obtained using RNAseq technology from the mammary gland of lactating cows receiving a low forage diet, supplemented or not with 4% sunflower oil. Among the 272 miRNAs characterized, eight were selected for RT-qPCR validations, showing the significant down-regulation of miR-142-5p and miR-20a-5p by sunflower supplementation. These two miRNAs are predicted to target genes whose expression was reported as differentially expressed by sunflower supplementation. Among their putative targets, ELOVL6 gene involved in lipid metabolism has been studied. However, a first analysis did not show its significant down-regulation, in response to the over-expression of miR-142-5p , of miR-20a-5p , or both, in a bovine mammary epithelial cell line. However, a clearer understanding of the miRNA expression by lipid supplementation would help to decipher the regulation of lactating cow mammary gland in response to nutrition.