Open AccessBone marrow basophils provide survival signals to immature B cells in vitro but are dispensable in vivo
Author(s) -
Joshua M. Moreau,
Selena Cen,
Alexandra Berger,
Caren Furlonger,
Christopher J. Paige
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0185509
Subject(s) - biology , bone marrow , microbiology and biotechnology , population , progenitor cell , cell , immunology , basophil , stem cell , b cell , medicine , genetics , immunoglobulin e , environmental health , antibody
Immature B cells are the first B cell progenitors to express a fully formed B cell receptor and are therefore subject to extensive selection processes that act to mitigate the emergence of autoreactive clones. While it is well appreciated that most B cell generation in the bone marrow is highly dependent on access to molecules present in the local milieu, the existence of extrinsically provided factors that modulate immature B cell biology is ambiguous. Nonetheless, a population of CD49b + CD90 lo cells has demonstrated in vitro potential to promote immature B cell survival. Using a mouse basophil reporter strain we confirmed the identity of these CD49b + CD90 lo supportive cells as basophils. However, analysis of bone marrow B cell populations following lineage specific basophil depletion demonstrates that basophils do not have a significant role in vivo in modulating immature B cell biology during steady-state conditions.