Open AccessThe role of PKA in the translational response to heat stress in Saccharomyces cerevisiae
Author(s) -
Carla Eliana Barraza,
Clara A. Solari,
Irina Marcovich,
Christopher J. Kershaw,
Fiorella Galello,
Silvia Rossi,
Mark P. Ashe,
Paula Portela
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0185416
Subject(s) - stress granule , eif4g , heat shock , microbiology and biotechnology , saccharomyces cerevisiae , translation (biology) , hsf1 , translational regulation , ribonucleoprotein , heat shock protein , cellular stress response , messenger rna , biology , chemistry , yeast , hsp70 , fight or flight response , genetics , gene , rna
Cellular responses to stress stem from a variety of different mechanisms, including translation arrest and relocation of the translationally repressed mRNAs to ribonucleoprotein particles like stress granules (SGs) and processing bodies (PBs). Here, we examine the role of PKA in the S . cerevisiae heat shock response. Under mild heat stress Tpk3 aggregates and promotes aggregation of eIF4G, Pab1 and eIF4E, whereas severe heat stress leads to the formation of PBs and SGs that contain both Tpk2 and Tpk3 and a larger 48S translation initiation complex. Deletion of TPK2 or TPK3 impacts upon the translational response to heat stress of several mRNAs including CYC1 , HSP42 , HSP30 and ENO2 . TPK2 deletion leads to a robust translational arrest, an increase in SGs/PBs aggregation and translational hypersensitivity to heat stress, whereas TPK3 deletion represses SGs/PBs formation, translational arrest and response for the analyzed mRNAs. Therefore, this work provides evidence indicating that Tpk2 and Tpk3 have opposing roles in translational adaptation during heat stress, and highlight how the same signaling pathway can be regulated to generate strikingly distinct physiological outputs.