Open AccessStudy of myelin structure changes during the nerve fibers demyelination
Author(s) -
N. N. Rodionova,
Elvin S. Allakhverdiev,
Г. В. Максимов
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0185170
Subject(s) - myelin , pronase , biophysics , nerve conduction velocity , chemistry , electrophysiology , anatomy , neuroscience , biochemistry , biology , central nervous system , trypsin , enzyme
Raman, NMR and EPR spectroscopy and electrophysiology methods were used to investigate the excitability and the packaging of myelin lipid layers and its viscosity during nerve exposure to pronase E. It was established that during exposure of nerve to pronase E the action potential (AP) conduction velocity and the Schwann cell (SC) (or myelin) water ordering increases, but the nerve myelin refractive index and internode incisions numbers decrease. This effect included two periods–short- and long-time period, probably, because the first one depends on SC protein changes and the second one–on the nerve fiber internode demyelination. It was concluded that high electrical resistance of myelin, which is important for a series of AP conduction velocity, not only depends on nerve fiber diameter and the myelin lipid composition, but also on the regularity of myelin lipid fatty acids and myelin lipid layer packing during the axoglial interaction.