Open AccessMultilocus genotyping of Giardia duodenalis in captive non-human primates in Sichuan and Guizhou provinces, Southwestern China
Author(s) -
Zhijun Zhong,
Yuan Tian,
Wei Li,
Huang Xiao-hong,
Lei Deng,
Suizhong Cao,
Yi Geng,
Hualin Fu,
Liuhong Shen,
Haifeng Liu,
Guangneng Peng
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0184913
Subject(s) - biology , multilocus sequence typing , genotype , genotyping , giardia , zoology , phylogenetic tree , feces , virology , ecology , genetics , gene
Giardia duodenalis is a common human and animal pathogen. It has been increasingly reported in wild and captive non-human primates (NHPs) in recent years. However, multilocus genotyping information for G . duodenalis infecting NHPs in southwestern China is limited. In the present study, the prevalence and multilocus genotypes (MLGs) of G . duodenalis in captive NHPs in southwestern China were determined. We examined 207 fecal samples from NHPs in Sichuan and Guizhou provinces, and 16 specimens were positive for G . duodenalis . The overall infection rate was 7.7%, and only assemblage B was identified. G . duodenalis was detect positive in northern white-cheeked gibbon (14/36, 38.9%), crab-eating macaque (1/60, 1.7%) and rhesus macaques (1/101, 0.9%). Multilocus sequence typing based on beta-giardin ( bg ), triose phosphate isomerase ( tpi ) and glutamate dehydrogenase ( gdh ) revealed nine different assemblage B MLGs (five known genotypes and four novel genotypes). Based on a phylogenetic analysis, one potentially zoonotic genotype of MLG SW7 was identified in a northern white-cheeked gibbon. A high degree of genetic diversity within assemblage B was observed in captive northern white-cheeked gibbons in Southwestern China, including a potentially zoonotic genotype, MLG SW7. To the best of our knowledge, this is the first report using a MLGs approach to identify G . duodenalis in captive NHPs in Southwestern China.