Open AccessA high mitochondrial transport rate characterizes CNS neurons with high axonal regeneration capacity
Author(s) -
Romain Cartoni,
Gülçin Pekkurnaz,
Chen Wang,
Thomas L. Schwarz,
Zhigang He
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0184672
Subject(s) - axoplasmic transport , regeneration (biology) , biology , axotomy , axon , neuroscience , nervous system , microbiology and biotechnology , mitochondrion , central nervous system , endosome , pten , signal transduction , pi3k/akt/mtor pathway , intracellular
Improving axonal transport in the injured and diseased central nervous system has been proposed as a promising strategy to improve neuronal repair. However, the contribution of each cargo to the repair mechanism is unknown. DRG neurons globally increase axonal transport during regeneration. Because the transport of specific cargos after axonal insult has not been examined systematically in a model of enhanced regenerative capacity, it is unknown whether the transport of all cargos would be modulated equally in injured central nervous system neurons. Here, using a microfluidic culture system we compared neurons co-deleted for PTEN and SOCS3, an established model of high axonal regeneration capacity, to control neurons. We measured the axonal transport of three cargos (mitochondria, synaptic vesicles and late endosomes) in regenerating axons and found that the transport of mitochondria, but not the other cargos, was increased in PTEN/SOCS3 co-deleted axons relative to controls. The results reported here suggest a pivotal role for this organelle during axonal regeneration.