Open AccessStrontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation
Author(s) -
Camille Ehret,
Rachida AidLaunais,
T. Sagardoy,
Robin Siadous,
Reine Bareille,
Sylvie Rey,
Stanislav Péchev,
Luc Etienne,
J. Kalisky,
Erwan de Monès,
Didier Letourneur,
Joëlle Amédée Vilamitjana
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0184663
Subject(s) - osteopontin , osteoid , strontium , in vivo , mineralization (soil science) , mesenchymal stem cell , chemistry , polysaccharide , matrix (chemical analysis) , extracellular matrix , in vitro , bone tissue , radiodensity , microbiology and biotechnology , nuclear chemistry , biophysics , biochemistry , biomedical engineering , anatomy , biology , medicine , endocrinology , surgery , chromatography , organic chemistry , nitrogen , radiography
Previous studies performed using polysaccharide-based matrices supplemented with hydroxyapatite (HA) particles showed their ability to form in subcutaneous and intramuscular sites a mineralized and osteoid tissue. Our objectives are to optimize the HA content in the matrix and to test the combination of HA with strontium (Sr-HA) to increase the matrix bioactivity. First, non-doped Sr-HA powders were combined to the matrix at three different ratios and were implanted subcutaneously for 2 and 4 weeks. Interestingly, matrices showed radiolucent properties before implantation. Quantitative analysis of micro-CT data evidenced a significant increase of mineralized tissue formed ectopically with time of implantation and allowed us to select the best ratio of HA to polysaccharides of 30% (w/w). Then, two Sr-substitution of 8% and 50% were incorporated in the HA powders (8Sr-HA and 50Sr-HA). Both Sr-HA were chemically characterized and dispersed in matrices. In vitro studies performed with human mesenchymal stem cells (MSCs) demonstrated the absence of cytotoxicity of the Sr-doped matrices whatever the amount of incorporated Sr. They also supported osteoblastic differentiation and activated the expression of one late osteoblastic marker involved in the mineralization process i . e . osteopontin. In vivo , subcutaneous implantation of these Sr-doped matrices induced osteoid tissue and blood vessels formation.