Open AccessMycoplasma-associated multidrug resistance of hepatocarcinoma cells requires the interaction of P37 and Annexin A2
Author(s) -
Danyang Liu,
Yang Hu,
Ying Guo,
Zhu Zhu,
Bingzheng Lu,
Xuelan Wang,
Yijun Huang
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0184578
Subject(s) - mycoplasma , mitoxantrone , multiple drug resistance , annexin a2 , annexin , biology , gemcitabine , microbiology and biotechnology , cancer cell , drug resistance , cancer research , virology , chemistry , cancer , flow cytometry , chemotherapy , genetics
Mycoplasma infection has been reported to be associated with cancer migration, invasion, epithelial-mesenchymal transition as well as the resistance to nucleoside analogues chemotherapeutic drugs. In this study, we found that the sensitivity of hepatocarcinoma cells to Cisplatin, Gemcitabine and Mitoxantrone was increased by mycoplasma elimination. Similar to the effect of anti-mycoplasma agent, interrupting the interaction between Mycoplasma hyorhinis membrane protein P37 and Annexin A2 of host cells using the N-terminal of ANXA2 polypeptide enhanced the sensitivity of HCC97L cells to Gemcitabine and Mitoxantrone. Meanwhile, we did not observe any changes in expression or distribution of multidrug resistance associated transporters, ATP-Binding Cassette protein B1, C1 and G2, on the removal of mycoplasma. These results suggest that mycoplasma induces a resistance to multiple drugs in hepatocarcinoma cells which required the interaction of P37 and Annexin A2. The pathway downstream this interaction needs to be explored.