Open AccessGeneration of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody
Author(s) -
Veronika Chromikova,
Maria A. Zaragoza,
Florian Krammer
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0183315
Subject(s) - monoclonal antibody , virology , virus , neutralization , biology , chinese hamster ovary cell , influenza a virus , cell culture , antibody , immunology , genetics
Because of the broad neutralization and in vivo protection across influenza A and influenza B virus strains, monoclonal antibody CR9114 is widely used in influenza virus research as a positive control in many experiments. To produce amounts sufficient for the demand requires regular transient transfections, resulting in varying yield as well as differing batch to batch quality. Here, we report the development of a serum-free CHO DG44 cell line, stably producing a CR9114-like antibody with a potential to become a useful influenza virus research tool.