Open AccessCloning and expression of genes encoding heat shock proteins in Liriomyza trifolii and comparison with two congener leafminer species
Author(s) -
Ya-Wen Chang,
Jingyun Chen,
MingXing Lu,
Yuan Gao,
Zi-Hua Tian,
WeiRong Gong,
Chunhong Dong,
YuZhou Du
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0181355
Subject(s) - biology , pest analysis , heat shock protein , gene , acclimatization , adaptation (eye) , botany , horticulture , genetics , neuroscience
The polyphagous agromyzid fly, Liriomyza trifolii , is a significant and important insect pest of ornamental and vegetable crops worldwide. The adaptation of insects to different environments is facilitated by heat shock proteins (HSPs), which play an important role in acclimation to thermal stress. In this study, we cloned and characterized five HSP-encoding genes of L . trifolii ( Lthsp20 , Lthsp40 , Lthsp60 , Lthsp70 , and Lthsp90 ) and monitored their expression under different thermal stresses using real-time quantitative PCR. Pupae of L . trifolii were exposed to 19 different temperatures ranging from -20 to 45°C. The results revealed that Lthsp20 , Lthsp40 , Lthsp70 and Lthsp90 were significantly upregulated in response to both heat and cold stress, while Lthsp60 was induced only by heat temperatures. The temperatures of the onset ( T on ) and maximal ( T max ) expression of the five Lthsps were also determined and compared with published T on and T max values of homologous genes in L . sativae and L . huidobrensis . Although L . trifolii occurs primarily in southern China, it has cold tolerance comparable with the other two Liriomyza species. Based on the heat shock proteins expression patterns, L . trifolii has the capacity to tolerate extreme temperatures and the potential to disseminate to northern regions of China.