Open AccessThe novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation
Author(s) -
Chunyan Zhang,
Cuifang Chang,
Weihua Zhao,
Hang Gao,
Qiwen Wang,
Deming Liu,
Fuchun Zhang,
Shifu Zhang,
Chi Xu
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0180607
Subject(s) - cell cycle , cell growth , gene knockdown , flow cytometry , rna interference , transfection , microbiology and biotechnology , biology , apoptosis , cell , cyclin d1 , cell culture , chemistry , gene , rna , genetics
Our previous study has proved that the chromosome 9 open reading frame 116 (C9orf116) ( NM_001106564.1 ) was significantly up-regulated in the proliferation phase of liver regeneration. To study its possible physiological function, we analyzed the effect of C9orf116 on BRL-3A cells via over-expression and interference technique. MTT results showed that the cell viability of the interference group was significantly lower than the control group at 48h after transfection ( P <0.05), whereas it was significantly higher in the over-expression group ( P <0.05). The flow cytometry results showed that C9orf116 knockdown or over-expression had little effect on BRL-3A cell apoptosis. However, the number of cells in division phase (G2/M) was significantly reduced in the interference group ( P <0.05), but significantly increased in the over-expression group ( P <0.01). Furthermore, the expressions of cell proliferation-related genes CCNA2, CCND1 and MYC both at mRNA and protein levels were down-regulated in the interference group and up-regulated in the over-expression group. Therefore, we concluded that C9orf116 may promote cell proliferation by modulating cell cycle transition and the expression of key genes CCNA2, CCND1 and MYC in BRL-3A cells.