Open AccessCold atmospheric plasma enhances osteoblast differentiation
Author(s) -
Kanako Tominami,
Hiroyasu Kanetaka,
Shota Sasaki,
Takayuki Mokudai,
Toshiro Kaneko,
Yoshimi Niwano
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0180507
Subject(s) - alkaline phosphatase , osteoblast , chemistry , irradiation , mineralization (soil science) , alizarin red , radical , biophysics , staining , biochemistry , in vitro , biology , enzyme , physics , organic chemistry , nuclear physics , nitrogen , genetics
This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining were applied to assess osteoblastic differentiation. The plasma-generated radicals were detected directly using an electron spin resonance-spin trapping technique. Results show that plasma irradiation under specific conditions increased ALP activity and enhanced mineralization, and demonstrated that the yield of radicals was increased in an irradiation-time-dependent manner. Appropriate plasma irradiation stimulated the osteoblastic differentiation of the cells. This process offers the potential of promoting bone regeneration.