Open AccessThree-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking
Author(s) -
Seth Langford,
Cody Wiggins,
Roque Santos,
Melinda Hauser,
Jeffrey M. Becker,
Arthur E. Ruggles
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0180503
Subject(s) - tracking (education) , particle (ecology) , positron emission , positron emission tomography , population , chemistry , physics , biophysics , biological system , materials science , biology , nuclear medicine , medicine , psychology , ecology , pedagogy , environmental health
A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18 F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. This work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.