Open AccessRNA sequencing demonstrates large-scale temporal dysregulation of gene expression in stimulated macrophages derived from MHC-defined chicken haplotypes
Author(s) -
Kristopher Irizarry,
Eileen Downs,
Randall Bryden,
Jory Clark,
Lisa Griggs,
Renee Kopulos,
Cynthia M. Boettger,
Thomas J. Carr,
Calvin L. Keeler,
Ellen W. Collisson,
Yvonne Drechsler
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0179391
Subject(s) - biology , haplotype , innate immune system , immune system , major histocompatibility complex , gene , rna , acquired immune system , immunology , gene expression , antigen presentation , immunity , microbiology and biotechnology , genetics , t cell , allele
Discovering genetic biomarkers associated with disease resistance and enhanced immunity is critical to developing advanced strategies for controlling viral and bacterial infections in different species. Macrophages, important cells of innate immunity, are directly involved in cellular interactions with pathogens, the release of cytokines activating other immune cells and antigen presentation to cells of the adaptive immune response. IFNγ is a potent activator of macrophages and increased production has been associated with disease resistance in several species. This study characterizes the molecular basis for dramatically different nitric oxide production and immune function between the B2 and the B19 haplotype chicken macrophages.A large-scale RNA sequencing approach was employed to sequence the RNA of purified macrophages from each haplotype group (B2 vs. B19) during differentiation and after stimulation. Our results demonstrate that a large number of genes exhibit divergent expression between B2 and B19 haplotype cells both prior and after stimulation. These differences in gene expression appear to be regulated by complex epigenetic mechanisms that need further investigation.