Open AccessAllele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae)
Author(s) -
Vivek Arora,
M. K. Ghosh,
Soumili Pal,
Gaurab Gangopadhyay
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0179189
Subject(s) - chalcone synthase , biology , genetics , locus (genetics) , moraceae , powdery mildew , gene , allele , cleaved amplified polymorphic sequence , molecular marker , flavonoid biosynthesis , genotype , population , genetic marker , single nucleotide polymorphism , botany , restriction fragment length polymorphism , gene expression , demography , transcriptome , sociology
Chalcone synthase (CHS) is an essential enzyme in the phenylpropanoid pathway that catalyzes the first step in flavonoid biosynthesis in plants under diverse environmental stress. We have used CHS as a candidate gene in mulberry and developed Single Nucleotide Polymorphism (SNP) based co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) marker associated with the CHS locus. The segregation pattern of the marker was studied in an F 1 population derived from a hybridization program between two mulberry genotypes showing polymorphism for the CHS locus. Differential CHS activity of the recombinants has been correlated with the segregation pattern of the marker. Homology modelling and docking studies are performed for both the identified CHS alleles and correlated with respective CHS activity. Phenotyping of Powdery Mildew infected F 1 population indicated a probable association with the CAPS marker.