Open AccessAcanthamoeba castellanii is not be an adequate model to study human adenovirus interactions with macrophagic cells
Author(s) -
Elodie Maisonneuve,
Estelle Cateau,
Nicolas Lévêque,
Sihem Kaaki,
Agnès BebyDefaux,
MarieHélène Rodier
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0178629
Subject(s) - acanthamoeba , amoeba (genus) , biology , protozoa , microbiology and biotechnology , in vitro , phagocyte , macrophage , virology , phagocytosis , genetics
Free living amoebae (FLA) including Acanthamoeba castellanii , are protozoa that feed on different microorganisms including viruses. These microorganisms show remarkable similarities with macrophages in cellular structures, physiology or ability to phagocyte preys, and some authors have therefore wondered whether Acanthamoeba and macrophages are evolutionary related. It has been considered that this amoeba may be an in vitro model to investigate relationships between pathogens and macrophagic cells. So, we intended in this study to compare the interactions between a human adenovirus strain and A . castellanii or THP-1 macrophagic cells. The results of molecular and microscopy techniques following co-cultures experiments have shown that the presence of the adenovirus decreased the viability of macrophages, while it has no effect on amoebic viability. On another hand, the viral replication occurred only in macrophages. These results showed that this amoebal model is not relevant to explore the relationships between adenoviruses and macrophages in in vitro experiments.