Open AccessInflammation-related microRNA expression level in the bovine milk is affected by mastitis
Author(s) -
YuChang Lai,
T. Fujikawa,
Tadashi Maemura,
Takaaki Ando,
Go Kitahara,
Yasuyuki Endo,
Osamu Yamato,
Masateru Koiwa,
Chikara Kubota,
Naoki Miura
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0177182
Subject(s) - mastitis , microrna , downregulation and upregulation , inflammation , real time polymerase chain reaction , biology , immunology , medicine , microbiology and biotechnology , genetics , gene
MicroRNA (miRNA) in tissue and liquid samples have been shown to be associated with many diseases including inflammation. We aimed to identify inflammation-related miRNA expression level in the bovine mastitis milk. Expression level of inflammation-related miRNA in milk from mastitis-affected and normal cows was analyzed using qPCR. We found that expression level of miR-21, miR-146a, miR-155, miR-222, and miR-383 was significantly upregulated in California mastitis test positive (CMT+) milk. We further analyzed these miRNA using a chip-based QuantStudio Digital PCR System. The digital PCR results correlated with those of qPCR, demonstrating upregulation of miR-21, miR-146a, miR-155, miR-222, and miR-383 in CMT+ milk. In conclusion, we identified miRNA that are upregulated in CMT+ milk. These miRNA exhibited sensitivity and specificity greater than 80% for differentiating between CMT+ milk and normal milk. Our findings suggest that inflammation-related miRNA expression level in the bovine milk was affected by mastitis, and miRNA in milk have potential for use as biomarkers of bovine mastitis.