Open Accessβ-Lapachone suppresses the lung metastasis of melanoma via the MAPK signaling pathway
Author(s) -
Ji Ye Kee,
Yo Han Han,
Dae Seung Kim,
Jeong Geon Mun,
Seong Hwan Park,
Hong Seob So,
Sung Joo Park,
Raekil Park,
Jae Young Um,
Seongin Hong
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0176937
Subject(s) - apoptosis , melanoma , cancer research , poly adp ribose polymerase , cell cycle , mapk/erk pathway , flow cytometry , metastasis , kinase , biology , viability assay , cell culture , chemistry , microbiology and biotechnology , cancer , polymerase , dna , biochemistry , genetics
β-Lapachone is a natural quinone compound from Lapacho trees, which has various pharmacological effects such as anti-bacterial, anti-fungal, anti-viral, and anti-inflammatory activities. However, the effect of β-lapachone on metastasis of melanoma cells is unclear. In this study, β-lapachone reduced cell viability of metastatic melanoma cancer cell lines B16F10 and B16BL6 through induction of apoptosis via the mitogen-activated protein kinase (MAPK) pathway. Additionally, flow cytometry results showed that β-lapachone increased DNA content in the G0/G1 phase of the cell cycle. Analysis of the mechanisms of these events indicated that β-lapachone regulated the expression of Bcl-2, Bcl-xL, and Bax, resulting in the activation of caspase-3, -8, -9, and poly-ADP-ribose polymerase (PARP). Moreover, the β-lapachone-administered group showed significantly decreased lung metastasis in the experimental mouse model. In conclusion, our study demonstrates the inhibitory effect of β-lapachone on lung metastasis of melanoma cells and provides a new insight into the role of β-lapachone as a potential antitumor agent.