Open AccessMultilocus genotyping of Enterocytozoon bieneusi derived from nonhuman primates in southwest China
Author(s) -
Zhijun Zhong,
Wei Li,
Lei Deng,
Yuan Shi,
Kui Wu,
Yuan Tian,
Huang Xiao-hong,
Yanchun Hu,
Hualin Fu,
Yi Geng,
Zhihua Ren,
Guangneng Peng
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0176926
Subject(s) - enterocytozoon bieneusi , genotyping , biology , microsporidiosis , genotype , multilocus sequence typing , zoonosis , virology , feces , phylogenetic tree , molecular epidemiology , genetics , microbiology and biotechnology , internal transcribed spacer , gene , microsporidia , spore
Enterocytozoon bieneusi has been increasingly reported in non-human primates (NHPs) in recent years, and this has garnered attention. However, reports of E . bieneusi infections in NHPs are limited worldwide. To appreciate the genetic diversity and assess the zoonotic potential during the transmission of human microsporidiosis, we examined a total of 369 fecal samples from NHPs and performed PCR amplification of the ITS gene of E . bieneusi . An infection rate of 12.5% (46/369) was detected in NHPs, with three known genotypes (D, PigEBITS7, and SC02) and a novel genotype (SCM01) characterized. Phylogenetic analysis indicated that all four genotypes in our study were classified as zoonotic group 1. Multilocus genotyping of positive E . bieneusi strains revealed that 36, 37, 30, and 29 specimens were successfully amplified and sequenced to generate 16, six, four, and five types of MS1, MS3, MS4, and MS7 loci, respectively. Twenty-four specimens were successfully amplified and sequenced at all four loci, forming 13 multilocus genotypes (MLGs). The occurrence of zoonotic genotypes suggests that zoonotic transmission of E . bieneusi between humans and NHPs has probably occurred and NHPs could be a source of human microspordiosis.