No serological evidence for Zika virus infection and low specificity for anti-Zika virus ELISA in malaria positive individuals among pregnant women from Madagascar in 2010 | Zendy

Norbert Georg Schwarz | Zendy; Eva Mertens | Zendy; Doris Winter | Zendy; Oumou MaïgaAscofaré | Zendy; Denise Dekker | Zendy; Stéphanie Jansen | Zendy; Dennis Tappe | Zendy; Njary Randriamampioa | Zendy; Jürgen May | Zendy; Raphaël Rakotozandrindrainy | Zendy; Jonas SchmidtChanasit | Zendy

Open Access

No serological evidence for Zika virus infection and low specificity for anti-Zika virus ELISA in malaria positive individuals among pregnant women from Madagascar in 2010

Author(s) -

Norbert Georg Schwarz,

Eva Mertens,

Doris Winter,

Oumou MaïgaAscofaré,

Denise Dekker,

Stéphanie Jansen,

Dennis Tappe,

Njary Randriamampioa,

Jürgen May,

Raphaël Rakotozandrindrainy,

Jonas SchmidtChanasit

Publication year - 2017

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0176708

Subject(s) - zika virus , virology , serology , malaria , biology , antibody , virus , medicine , immunology

It was previously reported that a malaria infection may interfere with the specificity of a commercial ELISA test against Zika virus (ZIKV). We analyzed 1,216 plasma samples from healthy, pregnant women collected in two sites in Madagascar in 2010 for ZIKV antibodies using a commercial ELISA and for Plasmodium infection by PCR. This screen revealed six putative ZIKV-positive samples by ELISA. These results could not be confirmed by indirect immunofluorescence assays or virus neutralization tests. Four of these six samples were also positive for P . falciparum . We noted that the frequency of malaria positivity was higher in ZIKV-ELISA positive samples (50% and 100% in the two study sites) than ZIKV-negative samples (17% and 10%, respectively), suggesting that malaria may have led to false ZIKV-ELISA positives.

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